第14回先端研究セミナーを2013年7月26日に開催しました。

演題:Cancer sensor/enrichment lab-on-a-chip without labeling via osmotic pressure-induced dielectrophoresis and New discovery method of enzyme-mimicking catalytic peptides for cancer drug synthesis.

演者:Hiroshi Matsui 博士(City University of New York – Hunter College, Department of Chemistry and Biochemistry, Professor)

日時:2013年7月26日(金) 16:00~17:00

場所:研究所3F 332号室

In the first part, a new lab-on-a-chip platform integrating electric cancer cell sensor and cancer cell separation on a micro-fabricated silicon chip is discussed. This sensing platform was designed to distinguish cells in deforming sizes and shapes by measuring their characteristic impedance signals on polysilicon microelectrodes. The swelling cells increase the impedance value selectively and the detection is made on the order of 5 cells/mL in less than 30 minutes. The aggressive breast cancer cells could be distinguished from less aggressive ones by measuring impedance values. After the detection, cancer cells can be selectively removed from the chip by applying negative dielectrophoresis due to distinguished dipole feature of swelling cancer cells. CTCs are rare, and the enrichment and the subsequent gene analysis of collected cancer cells are beneficial for future personal medicine.

In the second part, a new methodology that enables the selection of catalytic oligopeptides from sequence libraries based on their catalytic turnover is introduced. When a phage display library is exposed to precursors, phages that present catalytic sequences facilitate amide condensation and consequent localized gelation for panning. By using this approach, we generate the catalytic peptide library for synthesizing protease and Gonadotropin-releasing hormone (GnRH) analogues, useful for prostate cancer treatment. The production of cancer drugs with catalytic peptides in green synthetic process is valuable not only for the environment but also for designing a new delivery method of toxic drug by targeting them at specific body parts.

 

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